biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
EA-5, monoclonal
assay
≥95% (SDS-PAGE)
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Flow cytometry (5 µg/mL)
Frozen Sections
Immunoprecipitation
Paraffin Sections )
isotype
IgG1
shipped in
wet ice
Quality Level
target post-translational modification
unmodified
General description
Anti-CD40, mouse monoclonal, clone EA-5, recognizes ~44 kDa CD40 in Raji cells. It is validated for use in Flow Cytometry, immunoprecipitation, & immunohistochemistry on frozen & paraffin sections.
Protein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.
Recognizes the ~44 kDa CD40 protein in Raji cells.
Immunogen
transfected murine L cells expressing human CD40
Application
Flow cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.
Other Notes
FACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Fanslow, W.C., et al. 1992. J. Immunol.149, 655.
Law, C.L., et al. 1990. Leukemia4, 732.
Law, C.L., et al. 1990. Leukemia4, 732.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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