biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
38, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Cell Inhibition Assays (1-5 µg/mL)
Flow Cytometry (5 µg/mL)
Frozen Sections
Immunoprecipitation )
isotype
IgG2a
shipped in
wet ice
Quality Level
target post-translational modification
unmodified
General description
Anti-CD11a, mouse monoclonal, clone 38, recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 cells. It is validated for FC, IP, IHC on frozen sections and for cell inhibition assays.
Protein A purified mouse monoclonal antibody. Recognizes the I domain of the ~180 kDa integrin α L subunit (CD11a).
Recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 human tumor cells.
Application
Cell Inhibition Assays (1-5 µg/ml, see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
Molt-4 cells
Molt-4 cells
Other Notes
Also blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.
Holness, C.L., et al. 1995. J. Biol. Chem. 270, 877.
Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.
Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
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