219461
组织蛋白酶K，His•Tag®，人，重组，大肠埃希菌
Cathepsin K, His•Tag, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.
产品名称
组织蛋白酶K，His•Tag®，人，重组，大肠埃希菌, Cathepsin K, His•Tag, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥900 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
Disclaimer
毒性：标准处理（A）
General description
注意：1 mU = 1百万单元。
组织蛋白酶K是半胱氨酸蛋白酶木瓜蛋白酶超家族的成员，在破骨细胞介导的骨吸收和胶原降解中起重要作用。它主要由破骨细胞表达，最近的研究也表明它在成纤维细胞中表达。组织蛋白酶K活性异常，无论是太高还是太低，都会导致骨质减少性疾病，例如骨质疏松症症和致密性成骨不全症症。该重组酶包含C端His•Tag序列。
Other Notes
Delaisse, J.M., et al. 2003.Microsc.Res. Tech.61, 504.
Dodds, R.A.2003.Cell Biochem.Funct.21, 231.
Everts, V., et al. 2003.Calcif.Tissue Int.73, 380.
Hou, W.S., et al. 2003.Biol. Chem.384, 891.
Dodds, R.A.2003.Cell Biochem.Funct.21, 231.
Everts, V., et al. 2003.Calcif.Tissue Int.73, 380.
Hou, W.S., et al. 2003.Biol. Chem.384, 891.
一个单位定义为在37°C，pH 5.5下，每分钟水解0.4 µmol Z-FR-AMC的酶量。
Packaging
请参考特定浓度批号的标签。
Physical form
在50 mM NaOAC，pH 5.5中。通过添加10 mM MMTS停止激活，可以在还原条件下将其去除。
Preparation Note
初次解冻后，分装冻存（-70°C）。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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