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227036

Sigma-Aldrich

Cholera Toxin

from Vibrio cholerae, ≥95% (SDS-PAGE), solid, A-B type toxin, Calbiochem

别名:

Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free

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About This Item

CAS Number:
9012-63-9
MDL编号:
MFCD00130776
UNSPSC代码:
12352202
NACRES:
NA.51

Product Name

Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free, Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.

质量水平

100

描述

RTECS - LF3100000

方案

≥95% (SDS-PAGE)

表单

solid

制造商/商品名称

Calbiochem®

储存条件

do not freeze

溶解性

soluble (Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.)

运输

ambient

储存温度

2-8°C

相关类别

一般描述

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..
Useful for tissue culture applications.

生化/生理作用

Cell permeable: no
Primary Target
α-subunit of G-proteins
Product does not compete with ATP.
Reversible: no

包装

Please refer to vial label for lot-specific concentration.
yes

警告

Toxicity: Highly Toxic (H)

外形

Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.

重悬

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.
Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.

分析说明

Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.
single band by disc gel electrophoresis

其他说明

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.

法律信息

Not available for sale outside of the United States.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Skull and crossbones
GHS06

警示用语：

Danger

危险声明

H300 + H310,H315

危险分类

Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Skin Irrit. 2

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

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