biological source
goat
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting
Frozen Sections (1:4000)
Immunocytochemistry (1:200)
ELISA (1:10,000))
isotype
IgG
shipped in
ambient
Quality Level
target post-translational modification
unmodified
General description
Goat polyclonal antibody supplied as lyophilized undiluted serum. Recognizes cholera toxin B-subunit.
Recognizes the B-subunit of cholera toxin.
This Anti-Cholera Toxin, B-Subunit Goat pAb is validated for use in Immunoblotting, Frozen Sections, Immunocytochemistry, ELISA for the detection of Cholera Toxin, B-Subunit.
Immunogen
Vibrio cholerae
non-denatured Cholera Toxin, B-subunit
Application
Immunoblotting (see application references)
Frozen Sections (1:4000; see application references)
Immunocytochemistry (1:200; see application references)
ELISA (1:10,000; see application references)
Frozen Sections (1:4000; see application references)
Immunocytochemistry (1:200; see application references)
ELISA (1:10,000; see application references)
Physical form
Undiluted serum.
Preparation Note
Reconstitute in 100 µl dH₂O. Further dilute with aqueous buffers, such as PBS.
Other Notes
Antibody should be titrated for optimal results in individual systems.
Vadolas, J., et al. 1995. Eur. J. Immunol. 25, 969.
Craig, J.P. 1971. Microbial Toxins 2A, 189.
Selected Citations
Peters, I., et al. 2009. Biochim. Biophys. Acta1788, 964.
Balasubramanian, N., et al. 2007. Nature Cell Biology9, 1381.
Nemchinov, L.G., Et al. 2000. Arch. Virol.145, 2557.
Craig, J.P. 1971. Microbial Toxins 2A, 189.
Selected Citations
Peters, I., et al. 2009. Biochim. Biophys. Acta1788, 964.
Balasubramanian, N., et al. 2007. Nature Cell Biology9, 1381.
Nemchinov, L.G., Et al. 2000. Arch. Virol.145, 2557.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
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J Müthing et al.
Glycoconjugate journal, 14(1), 19-28 (1997-01-01)
In this study the comparative TLC immunostaining investigation of neutral GSLs and gangliosides from human skeletal and heart muscle is described. A panel of specific polyclonal and monoclonal antibodies as well as the GM1-specific choleragenoid were used for the overlay
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Annabel Guichard et al.
Cell host & microbe, 14(3), 294-305 (2013-09-17)
Cholera toxin (CT), a virulence factor elaborated by Vibrio cholerae, is sufficient to induce the severe diarrhea characteristic of cholera. The enzymatic moiety of CT (CtxA) increases cAMP synthesis in intestinal epithelial cells, leading to chloride ion (Cl(-)) efflux through
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