SCF-TrCP1 Protein Complex, Active, 10 µg

N-terminal GST-tagged, recombinant human Smurf1 full length, expressed in E coli.

The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCF^βTrCP1 mediates the ubiquitination and subsequent proteasomal degradation of a variety of proteins involved in various cellular processes, these include IκBα, an inhibitor of the transcription factor NFκB and β-catenin which is involved in the Wnt signalling pathway. A number of cancers, including those in breast, colon and pancreatic tissue have been associated with the deregulation of SCF^βTrCP1

Ubiquitination Cascade Comoponent: E3

Purified using glutathione sepharose.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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