Coagulation Factor XIII, Human Plasma

Native coagulation factor XIII from human plasma. Plasma Factor XIII is a tetramer (α₂β₂) composed of two identical α-subunits and two identical β-subunits. After thrombin cleavage converts the α₂β₂ tetramer to a modified α₂′β₂ tetramer, Ca²⁺ promotes both the dissociation of the β₂ dimer and the activation of the α₂′ dimer to its catalytically active form. This activated Factor XIII (Factor XIIIa) strengthens the fibrin clot by catalyzing the formation of glutamyl-lysine bonds between fibrin monomers.

In 500 µM EDTA, 50% glycerol.

Following initial thaw, aliquot and freeze (-20°C).

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Clotting activity is determined by comparison to a standard curve produced using normal pooled human plasma. One unit is defined as the activity in 1 ml of normal, pooled plasma.

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Toxicity: Standard Handling (A)