M.W. 23,000 (monomer); 115,000 (pentamer).

Native C-reactive protein highly purified from human serum. CRP is a major acute-phase plasma protein that is dramatically elevated in patients with acute-phase conditions. Composed of five identical noncovalently linked subunits. Exhibits Ca²⁺-dependent binding to a variety of substrates. Important in diagnosis of rheumatoid arthritis. Suitable for immunological studies. In purified states and in plasma the monomeric form (M.W. ~21 kDa) aggregates as a cyclic pentamer. Intended for use as a calibration standard for assays of CRP, for structural and functional studies, and as an antigen for production of antisera.Calibrated by electroimmunoassay against WHO reference standard or equivalent.

No reaction in ODD and Laurell electroimmunoassay against polyvalent antiserum to normal human serum, antiserum to pooled effusion fluids and monospecific antiserum to human IgG.

Toxicity: Standard Handling (A)

In 140 mM NaCl, 10 mM Tris, 2 mM CaCl₂, 0.1% NaN₃, pH 8.0.

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Following initial thaw, aliquot and freeze (-70°C).

Paffen, E., et al. 2004. Arterioscler. Thromb. Vasc. Biol.24, 975.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany