Creatine Phosphokinase-MM Isoenzyme, Human Skeletal Muscle

Native creatine phosphokinase-MM from human skeletal muscle. Catalyzes the reversible conversion of creatine and ATP to phosphocreatine and ADP. Plasma levels of CPK-MM are a useful indicator of acute myocardial infarction.

In 10 mM Sodium Chloride, 5 mM Succinate, 5 mM β-Mercaptoethanol, 1 mM EDTA, 50% Glycerol, pH 7.1 +/- 0.1.

Following initial thaw, aliquot and freeze (-20°C).

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Baral, R., et al. 1993. Intern. Med.33, 210.
Wu, A.H.B. 1992. Lab. Med.23, 303.
Bergmeyer, H.U. 1974. Methods Enzymatic Anal.2, 789.

One unit is defined as the amount of enzyme that will catalyze the formation of 1 µmol of ATP from ADP and creatine phosphate per min at 37°C, pH 7.4.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)