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251515-M

DAF-FM

Name: DAF-FM
Brand: MM

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

别名:

DAF-FM, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein

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关于此项目

经验公式（希尔记法）:

C₂₁H₁₄F₂N₂O₅

化学文摘社编号:

254109-20-1

分子量:

412.34

UNSPSC Code:

12352200

Assay:

≥98% (HPLC)

Form:

solution

Quality level:

100

Storage condition:

OK to freeze, protect from light

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属性

Quality Level

100

assay

≥98% (HPLC)

form

solution

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, protect from light

color

pale yellow

fluorescence

λ_ex ~500 nm, λ_em ~515 nm

storage temp.

−20°C

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

InChI key

DIJCILWNOLHJCG-UHFFFAOYSA-N

说明

General description

A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

Biochem/physiol Actions

Primary Target
Nitric oxide (NO) fluorescent indicator

Physical form

In DMSO.

Preparation Note

Following initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.

Other Notes

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew. Chem. Int. Ed.38, 3209.

Legal Information

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F

flash_point_c

87 °C

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全球贸易项目编号

货号	GTIN
251515-1MG	04055977199246
251515-1MG-M	04055977199246