251515-M
DAF-FM
A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
别名:
DAF-FM, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein
产品名称
DAF-FM, A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
InChI key
DIJCILWNOLHJCG-UHFFFAOYSA-N
InChI
1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
pale yellow
fluorescence
λex ~500 nm
λem ~515 nm
storage temp.
−20°C
Quality Level
Biochem/physiol Actions
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
Disclaimer
Toxicity: Irritant (B)
General description
A photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.
Legal Information
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew. Chem. Int. Ed.38, 3209.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew. Chem. Int. Ed.38, 3209.
Physical form
In DMSO.
Preparation Note
Following initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F
flash_point_c
87 °C
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