251520-M
DAF-FM DA
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
别名:
DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
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About This Item
经验公式（希尔记法）:
C25H18F2N2O7
CAS Number:
分子量:
496.42
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.25
质量水平
方案
≥98% (HPLC)
表单
solution
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
protect from light
颜色
pale yellow
荧光
λex ~500 nm
λem ~515 nm
储存温度
−20°C
SMILES字符串
Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC
InChI
1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
一般描述
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
生化/生理作用
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
警告
Toxicity: Irritant (B)
外形
A 5 mM solution in DMSO.
重悬
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
其他说明
Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.
法律信息
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
188.6 °F - closed cup - (Dimethylsulfoxide)
闪点(°C)
87 °C - closed cup - (Dimethylsulfoxide)
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