251520-M
DAF-FM DA
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.
别名:
DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
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关于此项目
经验公式（希尔记法）:
C25H18F2N2O7
化学文摘社编号:
分子量:
496.42
UNSPSC Code:
12352204
NACRES:
NA.25
MDL number:
SMILES string
Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC
InChI
1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
InChI key
BEVHTVRRVVEMEF-UHFFFAOYSA-N
assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
pale yellow
fluorescence
λex ~500 nm, λem ~515 nm
storage temp.
−20°C
Quality Level
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General description
A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515 ) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.
Biochem/physiol Actions
Primary Target
Nitric oxide (NO) fluorescent indicator
Nitric oxide (NO) fluorescent indicator
Physical form
A 5 mM solution in DMSO.
Preparation Note
Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.
Other Notes
Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.
Legal Information
Manufactured by Daiichi Pure Chemicals Co., Ltd
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - closed cup - (Dimethylsulfoxide)
flash_point_c
87 °C - closed cup - (Dimethylsulfoxide)
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