Detergent Variety Pack

A membrane protein is considered solubilized if it is present in the supernatant after one hour centrifugation of lysate or a homogenate at 100,000 x g. In most cases, it is also important that the biological activity of the protein be preserved in the supernatant after solubilization by a detergent. Hence, the appropriate detergent is that which yields the maximum amount of biological active protein in the supernatant. Given a large number of detergents available today, choosing an appropriate detergent can be a difficult process.

Five detergents with widely differing properties suitable for solubilization of membrane-bound proteins.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Multiple Toxicity Values, refer to MSDS (O)