Dipeptidyl Peptidase IV (CD26), Porcine Kidney

Native, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).

Please refer to vial label for lot-specific concentration.

In 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.

Dilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).

Bar, J., et al. 2003. Biol. Chem.384, 1553.
Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA100, 5063.
Ikehara, Y., et al. 1994. Methods. Enzymol.244, 215.
David, F., et al. 1993. J. Biol. Chem.268, 17247.
Thomsen, P.D., et al. 1993. Mamm. Genome4, 604.
Misumi, Y., et al. 1992. Biochim. Biophys. Acta.1131, 333.
Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler372, 213.
Checler, F., et al. 1985. J. Neurochem.45, 1509.
Imai, K., et al. 1983. J. Biochem.93, 431.
Kato, T., et al. 1979. Experientia.35, 409.
Kojima, K., et al. 1979. Anal. Biochem.100, 43.
Kato, T., et al. 1978. Biochem. Med.19, 351.
Kenny, A.J., et al. 1976. Biochem. J.157, 169.

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)