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324402

Sigma-Aldrich

Anti-Dopamine D₃ Receptor Rabbit pAb

lyophilized, Calbiochem®

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生物来源

rabbit

质量水平

抗体形式

serum

抗体产品类型

primary antibodies

克隆

polyclonal

形式

lyophilized

不包含

preservative

种属反应性

rat, human

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

同位素/亚型

IgG

运输

ambient

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

human ... DRD3(1814)
rat ... Drd3(29238)

一般描述

Anti-Dopamine D₃ Receptor, rabbit polyclonal, recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D3 dopamine receptor protein.
Recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors.

免疫原

a synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor

应用

ELISA (see comments)

Flow Cytometry (1:100, see application references)

Immunoblotting (1:500)

Immunohistochemistry (1:5000)

警告

Toxicity: Standard Handling (A)

重悬

Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

其他说明

This antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Jacqueline S Coley et al.
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Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 12(2), 353-370 (2017-01-31)
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