Anti-Dopamine D5 Receptor, rabbit polyclonal, recognizes the dopamine D5 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~49 kDa D₅ dopamine receptor protein.

Recognizes the dopamine D₅ receptor in rat brain. Does not cross-react with other dopamine receptors.

Human

a synthetic peptide (LPPGSNGTAYC) corresponding to amino acids 2-10 of human dopamine D₅ receptor, conjugated to carrier protein

ELISA (see comments)

Flow Cytometry (1:100, see application references)

Immunoblotting (1:200)

Immunohistochemistry (1:2500)

Undiluted serum.

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Positive Control
Rat brain

For immunohistochemistry, a 1:2500 dilution of the reconstituted antiserum demonstrated staining of specific cells in various regions of rat brain sections, including Cortex layer 5 and parafascicular nucleus. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

McKenna, F., et al. 2002. J. Neuroimmunol.132, 34.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)