324682
弹性蛋白酶，猪胰腺，高纯度，结晶
Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.
别名:
弹性蛋白酶，猪胰腺，高纯度，结晶
产品名称
弹性蛋白酶，猪胰腺，高纯度，结晶, Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.
description
Merck USA index - 14, 3535
form
lyophilized
specific activity
≥25 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
solubility
distilled water: 10 mg/mL
foreign activity
Chymotrypsin ≤0.2%
shipped in
ambient
storage temp.
−20°C
Quality Level
Disclaimer
毒性：标准处理（A）
General description
来自猪胰腺的天然弹性蛋白酶。一种丝氨酸蛋白酶，对包含白蛋白、酪蛋白、变性胶原、弹性蛋白、纤维蛋白和血红蛋白的蛋白质类和肽类（特别是在与中性氨基酸残基相邻的键处）以及许多含有天冬氨酸、苯丙氨酸或酪氨酸的合成底物的水解进行催化。被DFP，弹性蛋白和α2-巨球蛋白抑制。
来自猪胰腺的天然弹性蛋白酶。对包含白蛋白、酪蛋白、变性胶原、弹性蛋白、纤维蛋白和血红蛋白的蛋白质类和肽类（特别是在与中性氨基酸残基相邻的键处）以及许多含有天冬氨酸、谷氨酸、苯丙氨酸或酪氨酸的合成底物的水解进行催化。优先裂解具有小疏水侧链的氨基酸残基的羰基末端的肽键，例如甘氨酸，缬氨酸，亮氨酸，异亮氨酸，尤其是丙氨酸。被DFP，弹性蛋白和α2-巨球蛋白抑制。最佳pH为7.8-8.5；pI = 9.5。注意：弹性蛋白酶倾向于粘附在玻璃上。因此，建议使用硅化玻璃器皿。
Other Notes
Largman, C. et al. 1976.Biochemestry15, 2491.
Mandl, I. 1962.Methods in Enzymol.5, 665.
Mandl, I. 1962.Methods in Enzymol.5, 665.
一个单位定义为在25℃，pH值8.0下每分钟水解1.0 mol Suc-Ala-Ala-Pro-Abu-pNA （目录编号324699）的酶的量。
Physical form
从50 mM海藻糖，1 mM乙酸中冻干。
Preparation Note
复溶后，弹性蛋白酶可以在4°C，pH 6.0下储存以供长期使用。如果在室温下以其最佳pH值或接近其最佳pH值孵育，弹性蛋白酶会迅速自溶为肽混合物。储备溶液在4°C，pH 6.0下可稳定保存2个月。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 3
Yves Donati et al.
American journal of physiology. Lung cellular and molecular physiology, 318(4), L619-L630 (2020-02-06)
Mouse lung developmental maturation and final alveolarization phase begin at birth. During this dynamic process, alveolar cells modify their morphology and anchorage to the extracellular matrix. In particular, alveolar epithelial cell (AEC) type I undergo cytoplasmic flattening and folding to
Sanja Blaskovic et al.
American journal of physiology. Lung cellular and molecular physiology, 318(4), L606-L618 (2020-01-23)
Harmful consequences of cigarette smoke (CS) exposure during lung development can already manifest in infancy. In particular, early life exposure to nicotine, the main component of CS, was shown to affect lung development in animal models. We aimed to characterize
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