324717
Endoglycosidase H
Cleaves high-mannose N-glycans, suitable for Mass Spectrometry, from Streptomyces plicatus, recombinant, expressed in E. coli
别名:
内切糖苷酶H，皱襞链霉菌，重组，大肠埃希菌, 内切-β-N-乙酰氨基葡萄糖苷酶H，内切H
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关于此项目
化学文摘社编号:
MDL number:
NACRES:
NA.54
UNSPSC Code:
12352202
Specific activity:
≥3 units/mL, ≥30 units/mg protein
Recombinant:
expressed in E. coli
产品名称
内切糖苷酶H，皱襞链霉菌，重组，大肠埃希菌, Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.
recombinant
expressed in E. coli
conjugate
(N-linked)
form
liquid
specific activity
≥3 units/mL, ≥30 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected
shipped in
wet ice
Quality Level
General description
注意：1 mU = 1毫单位。
重组褶皱链霉菌内切糖苷酶H在大肠杆菌中表达。催化天冬酰胺连接的寡甘露糖和杂合寡糖的水解，但不催化来自糖蛋白的复合寡糖的水解。Endo H在寡糖的二乙酰基壳二糖核心中的两个N-乙酰基葡糖胺残基之间裂解。
Physical form
在50 mM NaCl，20 mM Tris-HCl，1 mM EDTA（pH 7.5）中。
Other Notes
Rao, V., et al. 1995.Structure 3, 449.
Trimble, R.B., et al. 1987.Methods Enzymol.138, 763.
Trumbly, R.J., et al. 1985.J. Biol. Chem.260, 5683.
Trimble, R.B., et al. 1987.Methods Enzymol.138, 763.
Trumbly, R.J., et al. 1985.J. Biol. Chem.260, 5683.
一个单位定义为在37°C，pH 5.5下每分钟催化从1.0 µmol变性核糖核酸酶B释放N-连接的寡糖的酶量。通过SDS-PAGE监测裂解。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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