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Merck
CN

33-017

HTRF®磷脂酰肌醇3激酶活性测定试剂盒，1920 孔

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 5 plates.

别名:

激酶活性检测

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UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

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产品名称

HTRF®磷脂酰肌醇3激酶活性测定试剂盒，1920 孔, The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 5 plates.

species reactivity

mouse, human

manufacturer/tradename

HTRF®
Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_006218.2

UniProt accession no.

P42336

detection method

fluorometric (HTRF)

shipped in

dry ice

Quality Level

100

Gene Information

human ... PIK3CA(5290)

Application

HTRF® PI 3激酶活性测定试剂盒（1920孔）用于检测磷脂酰肌醇3-激酶(PI3K)的活性。也在生化分析中用于检测LL-00071210（一种选择性PI3Kδ抑制剂）的效力和选择性。

Biochem/physiol Actions

PI3激酶催化磷脂酰肌醇3号位磷酸化。在ATP和Mg2+存在下，这种I类酶可催化磷脂酰肌醇4,5-二磷酸(PIP2)转化为磷脂酰肌醇3,4,5-三磷酸(PIP3)。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Features and Benefits

  • 大斯托克斯位移和时间分辨技术有助于减少物质干扰，尽可能减少假阴性问题
  • HTRF检测平台皮摩尔级的灵敏度可保留完整的酶动力学，获得生理相关性最强的检测参数。
  • 兼容所有I类磷脂酰肌醇3激酶(PI3K)同工型：方便进行相对数据比较，无需采用多种检测产品。
  • 成熟可靠的分析—采用来自密理博Upstate® KinaseProfiler激酶筛选服务的实践证明技术。
  • Z`因子通常超过0.8，保证可靠一致数据。

General description

磷脂酰肌醇3激酶活性检测试剂盒（HTRF均相时间分辨荧光法）是一种高性能的检测试剂盒，提供了一种以均相384孔形式测定全部I类PI3激酶的通用方法。

Other Notes

4X反应缓冲液

PIP2（底物；1mM储备液）

终止试剂A

终止试剂B

DM A检测试剂

DM B检测试剂

DM C检测试剂

Packaging

试剂盒容量：1920孔

Legal Information

HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

存储类别

10 - Combustible liquids

wgk

WGK 2

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Robert H Sinnamon et al.
Protein expression and purification, 73(2), 167-176 (2010-05-12)
Phosphoinositide 3-kinases have been targeted for therapeutic research because they are key components of a cell signaling cascade controlling proliferation, growth, and survival. Direct activation of the PI3Kalpha pathway contributes to the development and progression of solid tumors in breast
Marinela Méndez-Pertuz et al.
Nature communications, 8(1), 1278-1278 (2017-11-04)
Telomeres and the insulin/PI3K pathway are considered hallmarks of aging and cancer. Here, we describe a role for PI3K/AKT in the regulation of TRF1, an essential component of the shelterin complex. PI3K and AKT chemical inhibitors reduce TRF1 telomeric foci
Reine Nehmé et al.
Analytical and bioanalytical chemistry, 406(15), 3743-3754 (2014-05-13)
Human kinases are one of the most promising targets for cancer therapy. Methods able to measure the effects of drugs on these cell agents remain crucial for biologists and medicinal chemists. The current work therefore sought to develop an in-capillary

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