33-036
PI 3-Kinase HTRF® Assay; 10,000 wells
The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.
产品名称
PI 3-Kinase HTRF® Assay; 10,000 wells, The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.
species reactivity
mouse, human
manufacturer/tradename
HTRF®
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
detection method
fluorometric (HTRF)
shipped in
dry ice
Quality Level
Gene Information
human ... PIK3CA(5290)
Application
PI 3-Kinase HTRF® Assay; 10,000 wells has been used for measuring the enzymatic activity of the class I phosphoinositide (PI) 3-kinase isoforms by a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.
Biochem/physiol Actions
Target Sub-Family: PI3K/PI4K
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Phosphoinositide (PI) 3-kinase (PI3K) is a heterodimeric lipid kinase containing regulatory and catalytic subunits. It catalyzes the phosphorylation at the 3-position hydroxyl group of the inositol ring of phosphatidylinositol (PtdIns). PI3K is implicated in various cellular functions such as cell growth, proliferation, motility, differentiation, survival, apoptosis, cytoskeletal rearrangement, and intracellular trafficking. It also plays a vital role in glucose uptake triggered by insulin.
Other Notes
4X Reaction Buffer
PIP2 (Substrate; 1mM stock)
Stop Reagent A
Stop Reagent B
DM A Detection Reagent
DM B Detection Reagent
DM C Detection Reagent
PIP2 (Substrate; 1mM stock)
Stop Reagent A
Stop Reagent B
DM A Detection Reagent
DM B Detection Reagent
DM C Detection Reagent
Packaging
Kit capacity: 10,000 wells
Preparation Note
12 months at -70°C
Legal Information
HTRF is a registered trademark of Cis Bio International
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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