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化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352202
MDL number:
Specific activity:
≥30 units/mL, ≥75 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
form
liquid
specific activity
≥30 units/mL, ≥75 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
Proteases, none detected
shipped in
wet ice
Quality Level
General description
Recombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3- and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Physical form
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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