Recombinant, β1-3,6-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,3- and β1,6-linked galactose from complex carbohydrates and glycoproteins. Not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)