生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
2D3, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative (antibody only)
种属反应性
mouse, human
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
dilution
(ELISA (0.05 µg/mL)
Immunoblotting (0.5 µg/mL))
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pSer9)
基因信息
human ... GSK3B(2932)
mouse ... Gsk3B(56637)
一般描述
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
应用
ELISA (0.05 µg/ml)
Immunoblotting (0.5 µg/ml)
Immunoblotting (0.5 µg/ml)
外形
100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
制备说明
Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
分析说明
Positive Control
Pervanadate-treated HepG2 (included)
Pervanadate-treated HepG2 (included)
其他说明
For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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