biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
2D3, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative (antibody only)
species reactivity
mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA (0.05 µg/mL)
Immunoblotting (0.5 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer9)
Gene Information
human ... GSK3B(2932)
mouse ... Gsk3B(56637)
General description
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
Application
ELISA (0.05 µg/ml)
Immunoblotting (0.5 µg/ml)
Immunoblotting (0.5 µg/ml)
Physical form
100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
Preparation Note
Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
Analysis Note
Positive Control
Pervanadate-treated HepG2 (included)
Pervanadate-treated HepG2 (included)
Other Notes
For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable