361527
PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)
liquid, clone 2D3, Calbiochem®
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
2D3, monoclonal
Species reactivity:
mouse, human
Application:
—
Citations:
1
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
2D3, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative (antibody only)
species reactivity
mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA (0.05 µg/mL)
Immunoblotting (0.5 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer9)
Quality Level
Gene Information
human ... GSK3B(2932)
mouse ... Gsk3B(56637)
General description
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
Application
ELISA (0.05 µg/ml)
Immunoblotting (0.5 µg/ml)
Immunoblotting (0.5 µg/ml)
Physical form
100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
Preparation Note
Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
Analysis Note
Positive Control
Pervanadate-treated HepG2 (included)
Pervanadate-treated HepG2 (included)
Other Notes
For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Adam R Cole et al.
Methods in molecular biology (Clifton, N.J.), 468, 45-65 (2008-12-23)
Glycogen synthase kinase (GSK)-3 is a key signalling intermediate in the action ofWnts. This protein kinase is ubiquitously expressed and has high inherent activity but is inhibited by activation of Wnt signalling or activation of growth factor receptor tyrosine kinases
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