Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.

In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.

Fan, Z., et al. 2002. Mol. Cell. Biol.22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol.20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem.276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol.244, 68.

One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)