Dkk-1, His•Tag^®, Mouse, Recombinant

Dkk-1, His•Tag, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells.

Recombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.

ND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.

Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.

Lyophilized from 0.2 µm filtered PBS, BSA.

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

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CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Regulatory Review (Z)