371207
Dkk-1, Mouse, Recombinant
Dkk-1, Mouse, Recombinant, consists of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Form:
liquid
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in CHO cells
recombinant
expressed in CHO cells
assay
≥95% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
Quality Level
General description
Recombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND50 = 0.02-0.1 µg/ml.
Biochem/physiol Actions
ND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-inducedalkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.
Physical form
Lyophilized from 0.2 µm sterile-filtered PBS, BSA.
Preparation Note
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in PBS containing at least 0.1% human or bovine serum albumin.
Other Notes
Pinzone, J.J., et al. 2009. Blood113, 517.
Li, J., et al. 2006. Bone36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C., 2006. Oncogene25, 7469.
Verani, R., et al. 2006. J. Neurochem.101, 242.
Cappuccio, I., et al. 2005. J. Neurosci.25, 2647.
Kemp, C., et al. 2005. Dev. Dyn.233, 1064.
Adamska, M., et al. 2004. Dev. Biol.272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol.65, 582.
Mao, B., et al. 2002. Nature417, 664.
Mao, B., et al. 2001. Nature411, 321.
Glinka, A., et al. 1998. Nature391, 357.
Li, J., et al. 2006. Bone36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C., 2006. Oncogene25, 7469.
Verani, R., et al. 2006. J. Neurochem.101, 242.
Cappuccio, I., et al. 2005. J. Neurosci.25, 2647.
Kemp, C., et al. 2005. Dev. Dyn.233, 1064.
Adamska, M., et al. 2004. Dev. Biol.272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol.65, 582.
Mao, B., et al. 2002. Nature417, 664.
Mao, B., et al. 2001. Nature411, 321.
Glinka, A., et al. 1998. Nature391, 357.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 3
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