Anti-G_sα-Subunit, C-Terminal (385-394) Rabbit pAb

Anti-G_sα-Subunit, C-Terminal (385-394), rabbit polyclonal, recognizes both large and small forms of G_sα-subunit. It is validated for use in Western blotting.

Protein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa G_sα subunit protein.

Recognizes both large and small forms of G_sα-subunit. Does not cross-react with G_iα-1-, G_iα-2-, G_iα-3-, or G_oα-subunits.

a synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian G_sα subunit, conjugated to KLH

Immunoblotting (1:1000)

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C).

Positive Control
G_sα-Subunit, His•Tag, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or G_sα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)

Does not cross-react with G_iα-1, G_iα-2, G_iα-3, or G_oα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for G_sα, G_iα-1, G_Iα-2, G_iα-3, and G_oα. Variables associated with assay conditions will dictate the proper working dilution.

Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Raymond, J.R., et al. 1993. Biochemistry32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)