371732
Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb
liquid, Calbiochem®
别名:
Anti-Gₛα Antibody, Gₛα-Subunit Detection Antibody, Rabbit Anti-Gₛα-Subunit
产品名称
Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb, liquid, Calbiochem®
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1:1000))
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GNAI1(2770)
Analysis Note
Positive Control
Gsα-Subunit, His•Tag, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)
Gsα-Subunit, His•Tag, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)
Application
Immunoblotting (1:1000)
Disclaimer
Toxicity: Standard Handling (A)
General description
Anti-Gsα-Subunit, C-Terminal (385-394), rabbit polyclonal, recognizes both large and small forms of Gsα-subunit. It is validated for use in Western blotting.
Protein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa Gsα subunit protein.
Recognizes both large and small forms of Gsα-subunit. Does not cross-react with Giα-1-, Giα-2-, Giα-3-, or Goα-subunits.
Immunogen
a synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH
Other Notes
Does not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
Kumar, R., et al. 1994. J. Mol. Cell. Cardiol.26, 1537.
Raymond, J.R., et al. 1993. Biochemistry32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Raymond, J.R., et al. 1993. Biochemistry32, 11064.
Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Physical form
In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Pasteurella multocida toxin (PMT) is a virulence factor responsible for the pathogenesis of some Pasteurellosis. PMT exerts its toxic effects through the activation of heterotrimeric GTPase (G(q), G(12/13) and G(i))-dependent pathways, by deamidating a glutamine residue in the α subunit
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