跳转至内容
Merck
CN
登录查看公司和协议定价

About This Item

UNSPSC代码:
12352203
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

生物来源

rabbit

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

polyclonal

表单

liquid

包含

≤0.1% sodium azide as preservative

种属反应性

human, canine, mouse, rat, porcine, bovine

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)

一般描述

Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP3 receptor protein.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Rabbit pAb is validated for use in ELISA, FC, Immunoblotting, IF, IHC, IP, Radioimmunoassay for the detection of IP₃ Receptor.

免疫原

a synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor

应用

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:250)

Immunofluorescence (1:200)

Immunohistochemistry (1:200)

Immunoprecipitation (1:200)

Radioimmunoassay (1:10000)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-20°C).

其他说明

Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Variables associated with assay conditions will dictate the proper working dilution.



Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies



NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.



• TBST

• 20 mM Tris-HCl, pH 7.6

• 150 mM NaCl

• 0.05% Tween®-20 detergent



1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).

2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.

3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.

4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.

5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.

6. Shake for 1 h.

7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.

8. Mix development solution (1:1) (Amersham ECL kit).

9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

未找到合适的产品?  

试试我们的产品选型工具.

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持