407143-M
Anti-IP3 Receptor Rabbit pAb
liquid, Calbiochem®
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human, canine, mouse, rat, porcine, bovine
Application:
—
Citations:
-
biological source
rabbit
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, canine, mouse, rat, porcine, bovine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)
General description
Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP3 receptor protein.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Rabbit pAb is validated for use in ELISA, FC, Immunoblotting, IF, IHC, IP, Radioimmunoassay for the detection of IP₃ Receptor.
Immunogen
a synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
Application
ELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:250)
Immunofluorescence (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Radioimmunoassay (1:10000)
Flow Cytometry (1:1000)
Immunoblotting (1:250)
Immunofluorescence (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Radioimmunoassay (1:10000)
Physical form
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Variables associated with assay conditions will dictate the proper working dilution.
Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies
NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.
• TBST
• 20 mM Tris-HCl, pH 7.6
• 150 mM NaCl
• 0.05% Tween®-20 detergent
1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
6. Shake for 1 h.
7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
8. Mix development solution (1:1) (Amersham ECL kit).
9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies
NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.
• TBST
• 20 mM Tris-HCl, pH 7.6
• 150 mM NaCl
• 0.05% Tween®-20 detergent
1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
6. Shake for 1 h.
7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
8. Mix development solution (1:1) (Amersham ECL kit).
9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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