Recombinant, rat insulin degrading enzyme fused to a His•Tag sequence and expressed in S. frugiperda insect cells. Insulin degrading enzyme (Insulysin) is a metalloprotease that degrades insulin and a variety of other peptides, including glucagon, insulin-like growth factors I and II, atrial natriuretic peptide, TGF-α, and amyloid peptides. Reported to be inversely correlated with brain Aβ peptide levels in insulysin-deficient mice compared to wild-type mice.

Please refer to vial label for lot-specific concentration.

In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.

Following initial thaw, aliquot and freeze (-70°C).

Hersh, L.B. 2003. Curr. Pharm. Des.9, 449.
Miller, B.C., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6221.
Song, E.S., et al. 2003. J. Biol. Chem.278, 49789.
Mukherjee, A., and Hersh, L.B. 2002. J. Alzheimer′s Dis.4, 341.
Song, E.S., et al. 2001. J. Biol. Chem.276, 1152.
Becker, A.B., and Roth, R.A. 1995. Methods Enzymol.248, 693.

One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)