Insulin-Like Growth Factor-II, Human, Recombinant, E. coli

Recombinant, human insulin-like growth factor-II expressed in E. coli. A growth-promoting peptide that is structurally related to insulin. IGF-II shares about 76% sequence identity with IGF-I and ~50% with pro-insulin. Its actions are similar to IGF-I (Cat. No. 407240). IGF-II is a nonglycosylated, single chain peptide of 67 amino acids. Plays an important role in fetal development. Stimulates muscle and bone cell proliferation and differentiation.

ED₅₀ = 5-10 ng/ml

Lyophilized from sterile-filtered acetic acid.

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA. Avoid using buffers near the isoelectric point (6.5).

Feldser, D., et al. 1999. Cancer Res.59, 3915.
Hill, P.A., et al. 1995. Endocrinology136, 124.
Nielson, F.C., et al. 1995. Nature 377, 358.
LeRoith, D., and Roberts, C.R., Jr. 1993. Ann. N.Y. Acad. Sci.692, 1.
Lowe, W.L. 1991. In Insulin-Like Growth Factors: Molecular and Cellular Aspects (LeRoith, D., Ed.) CRC Press Inc., Boca Raton p. 49.
Rotwein, P. 1991. Growth Factors5, 3.

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Toxicity: Standard Handling (A)