assay
≥97% (SDS-PAGE)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
<1.0 EU/μg protein Endotoxin
solubility
soluble (Reconstitute to 100 µg/ml in sterile PBS.)
shipped in
ambient
storage temp.
−20°C
Quality Level
General description
Recombinant, mouse insulin-like growth factor binding protein-related protein 1 (IGFBP-rp1) consisting of amino acids 28-281 and expressed in S. frugiperda 21 cells with an N-terminal His•Tag. Mouse IGFBP-rp1 cDNA encodes a 281 amino acid precursor protein which contains a putative 25 aa signal peptide and a 256 aa mature region. IGFBP modifies affinities of the binding proteins to IGF.
Biochem/physiol Actions
Functional ELISA Immobilized rmIGFBP-rp1/IGFBP-7 (500 ng/ml) produces 50% optimal binding to rhCCL21/6Ckine at 4-20 ng/ml.
Physical form
Lyophilized from 0.2 µm sterile-filtered PBS.
Preparation Note
Reconstitute to 100 µg/ml in sterile PBS.
Other Notes
Ahmed, S. et al. 2003. Biochem. Biophys. Res. Commun.310, 612.
Nagakubo, D. et al. 2003. J. Immunol.171, 553.
Wilson, HM. et al. 2002. Cell Growth Differ.13, 205.
Degeorges, A. et al. 2000. J. Histochem. Cytochem.48, 747.
Kato, M.V. 2000. Mol. Med.6, 126.
Hwa, V. et al. 1999. Endocr. Rev.20, 761.
Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA94,12981.
Kato, M.V. et al. 1996. Oncogene12, 1361.
Oh, Y. et al. 1996. J. Biol. Chem.271, 30322.
Nagakubo, D. et al. 2003. J. Immunol.171, 553.
Wilson, HM. et al. 2002. Cell Growth Differ.13, 205.
Degeorges, A. et al. 2000. J. Histochem. Cytochem.48, 747.
Kato, M.V. 2000. Mol. Med.6, 126.
Hwa, V. et al. 1999. Endocr. Rev.20, 761.
Kim, HS. et al. 1997. Proc. Natl. Acad. Sci. USA94,12981.
Kato, M.V. et al. 1996. Oncogene12, 1361.
Oh, Y. et al. 1996. J. Biol. Chem.271, 30322.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 1
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