Interleukin-6, Human, Recombinant, E. coli

Research area: Immunology and Cytokines

Human sequence IL-6 is active in murine cells.1 µg is equivalent to 7.0 x 10³ WHO units. 8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.

Interleukin-6has been used:

• to study IL-6 effects on lung cancer-derived mesenchymal stromalcells (MSCs) and to test the ability of statins to block these effects.
• toinvestigate the role of IL-6 in the development of coronary artery spasm (CAS).
• to study the effects of IL-6 on the bioactivity of therapeutic interferon (IFN)variants.

Interleukin-6 (IL-6) is a prototypic pleiotropic, pro-inflammatory cytokine that is expressed in response to injury, inflammation, and infection.(5) IL-6 triggers the activation of three distinct pathways: signal transducer and activator of transcription 3 (STAT-3), extracellular signal-regulated kinase 1 and 2 (Erk1/2), and protein kinase b/protein kinase b-alpha (Akt). Consequently, this activation leads to the upregulation of numerous genes that impact essential cellular processes including survival, proliferation, differentiation, osteogenesis/osteolysis, angiogenesis, and immune modulation. It stimulates the production of acute-phase proteins by hepatocytes. IL-6 is also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B cells, T cells, and hepatocytes. Stimulates growth and inhibits constitutive, protein synthesis-independent apoptosis of murine B cell hybridoma 7TD1. IL-6 is also considered as a potential marker of adipocyte differentiation. Interleukin-6 is a protein produced by a variety of cell types including T-lymphocytes, monocytes, diploid fibroblasts, hepatocytes, cardiac myxomas, certain cervical cancer cells, bladder cell carcinomas, vascular endothelial cells, and human myelomas. It has a variety of biological effects and does not show a high degree of species specificity.

Carrier free.

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to 0.1-1.0 mg/ml in 100 mM acetic acid.

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Toxicity: Standard Handling (A)