assay
≥95% (SDS-PAGE)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
cell culture | mammalian: suitable
impurities
≤1.0 EU/μg Endotoxin (EU/μg IL-6)
solubility
soluble (Reconstitute to 0.1-1.0 mg/ml in 100 mM acetic acid.)
UniProt accession no.
shipped in
ambient
storage temp.
−70°C
Quality Level
Gene Information
human ... IL6(3569)
Application
Interleukin-6has been used:
- to study IL-6 effects on lung cancer-derived mesenchymal stromalcells (MSCs) and to test the ability of statins to block these effects.
- toinvestigate the role of IL-6 in the development of coronary artery spasm (CAS).
- to study the effects of IL-6 on the bioactivity of therapeutic interferon (IFN)variants.
Biochem/physiol Actions
Interleukin-6 (IL-6) is a prototypic pleiotropic, pro-inflammatory cytokine that is expressed in response to injury, inflammation, and infection.(5) IL-6 triggers the activation of three distinct pathways: signal transducer and activator of transcription 3 (STAT-3), extracellular signal-regulated kinase 1 and 2 (Erk1/2), and protein kinase b/protein kinase b-alpha (Akt). Consequently, this activation leads to the upregulation of numerous genes that impact essential cellular processes including survival, proliferation, differentiation, osteogenesis/osteolysis, angiogenesis, and immune modulation. It stimulates the production of acute-phase proteins by hepatocytes. IL-6 is also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B cells, T cells, and hepatocytes. Stimulates growth and inhibits constitutive, protein synthesis-independent apoptosis of murine B cell hybridoma 7TD1. IL-6 is also considered as a potential marker of adipocyte differentiation. Interleukin-6 is a protein produced by a variety of cell types including T-lymphocytes, monocytes, diploid fibroblasts, hepatocytes, cardiac myxomas, certain cervical cancer cells, bladder cell carcinomas, vascular endothelial cells, and human myelomas. It has a variety of biological effects and does not show a high degree of species specificity.
Disclaimer
Toxicity: Standard Handling (A)
General description
Research area: Immunology and Cytokines
Human sequence IL-6 is active in murine cells.1 µg is equivalent to 7.0 x 103 WHO units. 8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.
Human sequence IL-6 is active in murine cells.1 µg is equivalent to 7.0 x 103 WHO units. 8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.
Other Notes
Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9.
Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890.
Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182.
Liu, J., et al. 1994. Cell Immunol.155, 229.
Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.
Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890.
Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182.
Liu, J., et al. 1994. Cell Immunol.155, 229.
Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.
Physical form
Carrier free.
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to 0.1-1.0 mg/ml in 100 mM acetic acid.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
监管及禁止进口产品
此项目有
Louise Larocque et al.
Journal of biomedicine & biotechnology, 2011, 174615-174615 (2011-03-16)
The traditional antiviral assays for the determination of interferon potency are reported to have considerable variability between and within assays. Although several reporter gene assays based on interferon-inducible promoter activities have been reported, data from comprehensive validation studies are lacking
Johnathan Ho et al.
PLoS biology, 14(10), e2000117-e2000117 (2016-10-26)
STAT2 is the quintessential transcription factor for type 1 interferons (IFNs), where it functions as a heterodimer with STAT1. However, the human and murine STAT2-deficient phenotypes suggest important additional and currently unidentified type 1 IFN-independent activities. Here, we show that
Sabine Galland et al.
Cancer letters, 484, 50-64 (2020-05-19)
Solid tumor growth triggers a dynamic host response, which recapitulates wound healing and defines the tumor microenvironment (TME). In addition to the action of the tumor cells themselves, the TME is maintained by a myriad of immune and stromal cell-derived
Yen-Kuang Lin et al.
Oxidative medicine and cellular longevity, 2022, 9964689-9964689 (2022-02-01)
Apolipoprotein (a)/lipoprotein(a) (Lp(a)), a major carrier of oxidized phospholipids, and α7-nicotinic acetylcholine receptor (α7-nAChR) may play an important role in the development of coronary artery spasm (CAS). In CAS, the association between Lp(a) and the α7-nAChR-modulated inflammatory macrophage polarization and
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