方案
≥97% (SDS-PAGE)
质量水平
表单
lyophilized
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
杂质
≤1 EU/μg Endotoxin (EU/μg IL-6)
运输
ambient
储存温度
−70°C
一般描述
Recombinant, mouse interleukin-6 expressed in E. coli. Stimulates the production of acute phase proteins by hepatocytes. Induces growth and differentiation of B cells, hepatocytes, keratinocytes, and nerve cells. Does not inhibit any significant activity on human cells.
生化/生理作用
ED₅₀ = 20-60 pg/ml as measured in a cell proliferation assay with a factor-dependent plasmacytoma cell line
外形
Lyophilized from sterile-filtered 50 mM NaOAc, 1 mM EDTA, 5% trehalose, 50 µg BSA/µg IL-6 pH 4.0.
制备说明
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
其他说明
Matsuda, T., et al. 1994. Biochem. Biophys. Res. Commun.202, 637.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Irritant (B)
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
T Matsuda et al.
Biochemical and biophysical research communications, 202(1), 637-642 (1994-07-15)
Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein gp130, which associates with the IL-6 receptor alpha chain (IL-6R alpha) in the presence of IL-6. We prepared monoclonal antibodies (mAbs) specific to murine gp130 by immunizing rats and hamsters with
Marta Kisgati et al.
Cytotechnology, 54(1), 5-14 (2008-11-13)
Retroviral gene transfer and bone marrow transplantation has been used by many investigators to study the role of macrophage proteins in different mouse models of human disease. While this approach is faster and less expensive than generating transgenic mice with
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