Recombinant, mouse interleukin-6 expressed in E. coli. Stimulates the production of acute phase proteins by hepatocytes. Induces growth and differentiation of B cells, hepatocytes, keratinocytes, and nerve cells. Does not inhibit any significant activity on human cells.

ED₅₀ = 20-60 pg/ml as measured in a cell proliferation assay with a factor-dependent plasmacytoma cell line

Lyophilized from sterile-filtered 50 mM NaOAc, 1 mM EDTA, 5% trehalose, 50 µg BSA/µg IL-6 pH 4.0.

Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Matsuda, T., et al. 1994. Biochem. Biophys. Res. Commun.202, 637.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Irritant (B)