Interleukin-12, Human, Recombinant, S. frugiperda

Recombinant, human interleukin-12 expressed in S. frugiperda insect cells. Pleiotropic cytokine that is produced by monocytes/macrophages, B cells, and connective tissue-type mast cells. Also known as natural killer cell stimulatory factor and cytotoxic lymphocyte maturation factor. A potent inducer of IFN-γ production and T cell differentiation and function.

ED₅₀ = 50-200 pg/ml as measured by its ability to stimulate the proliferation of PHA-activated human lymphoblasts

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-12.

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to a concentration ≥1 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

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Gubler, U., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 4143.
Stern, A.S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 6808.
Kobayashi, M., et al. 1989. J. Exp. Med. 170, 827.

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Toxicity: Standard Handling (A)