生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
mouse, human
请勿与下列物质发生反应
rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
wet ice
储存温度
−70°C
靶向翻译后修饰
phosphorylation (pTyr1022/pTyr1023)
基因信息
human ... JAK1(3716)
一般描述
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK1 protein phosphorylated on Tyr1022/1023.
Recognizes the ~130 kDa phosphorylated JAK1 in LIF-treated 3T3-L1 cells. Does not cross-react with other JAKs or related kinases.
This PhosphoDetect Anti-JAK1 (pTyr1022/1023) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK1 (pTyr1022/1023).
免疫原
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)
应用
Immunoblotting (1:1000)
外形
In Dulbecco′s PBS (without Ca2+ and Mg2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
制备说明
Following intitial thaw, aliquot and freeze (-70°C).
分析说明
Positive Control
3T3L1 cells treated with LIF
3T3L1 cells treated with LIF
其他说明
A ~110 kDa degradation product is also visible in some cell or tissue preparations. Does not cross-react with any other JAK proteins. Immunogen sequence is conserved in pig JAK1, but cross-reactivity has not been tested. When used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the optimal dilution.
Liu, K.D., et al. 1997. Curr. Biol.7, 817.
Gauzzi, M.C., et al. 1996. J. Biol. Chem.271, 20494.
Gauzzi, M.C., et al. 1996. J. Biol. Chem.271, 20494.
法律信息
Manufactured by Biosource International.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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