biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human
should not react with
rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
phosphorylation (pTyr1022/pTyr1023)
Quality Level
Gene Information
human ... JAK1(3716)
General description
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~130 kDa JAK1 protein phosphorylated on Tyr1022/1023.
Recognizes the ~130 kDa phosphorylated JAK1 in LIF-treated 3T3-L1 cells. Does not cross-react with other JAKs or related kinases.
This PhosphoDetect Anti-JAK1 (pTyr1022/1023) Rabbit pAb is validated for use in Immunoblotting for the detection of JAK1 (pTyr1022/1023).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰²²and Tyr¹⁰²³ phosphorylation sites of human JAK1 (Janus Kinase 1)
Application
Immunoblotting (1:1000)
Physical form
In Dulbecco′s PBS (without Ca2+ and Mg2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
Preparation Note
Following intitial thaw, aliquot and freeze (-70°C).
Analysis Note
Positive Control
3T3L1 cells treated with LIF
3T3L1 cells treated with LIF
Other Notes
A ~110 kDa degradation product is also visible in some cell or tissue preparations. Does not cross-react with any other JAK proteins. Immunogen sequence is conserved in pig JAK1, but cross-reactivity has not been tested. When used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. Variables associated with assay conditions will dictate the optimal dilution.
Liu, K.D., et al. 1997. Curr. Biol.7, 817.
Gauzzi, M.C., et al. 1996. J. Biol. Chem.271, 20494.
Gauzzi, M.C., et al. 1996. J. Biol. Chem.271, 20494.
Legal Information
Manufactured by Biosource International.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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