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Merck
CN

422426

KGF/FGF-7, Mouse, Recombinant

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关于此项目

NACRES:
NA.77
UNSPSC Code:
12352202
Form:
solid
Assay:
≥90% (SDS-PAGE)
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assay

≥90% (SDS-PAGE)

Quality Segment

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

<1.0 EU/μg protein Endotoxin

shipped in

ambient

storage temp.

−20°C

General description

Recombinant, mouse keratinocyte growth factor (KGF) consisting of amino acids 32-194 and expressed in E. coli. KGF plays an essential role in development, tumorigenesis, angiogenesis, morphogenesis, and wound healing. Mature mouse KGF amino acid sequence is 96% identical with rat, bovine, ovine, equine, and human forms and is active across species.

Biochem/physiol Actions

ED₅₀ = 10-50 ng/ml as measured in a cell proliferation assay using 4MBr-5 monkey epithelial cells.

Physical form

Lyophilized from 0.2 µm sterile-filtered MOPS, Na₂SO₄, EDTA, DTT BSA.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 250 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Belleudi, F., et al. 2007. Traffic8, 1854.
Finch, P.W. and J.S. Rubin. 2006. J. Natl. Cancer Inst.98, 812.
de Georgi, V., et al. 2007. Dermatol. Clin.25, 477.
Niu, J., et al. 2007. J. Biol. Chem.282, 6001.
Werner, S., et al. 2007. J. Invest. Dermatol.127, 998.
Cardinali, G., et al. 2005. J. Invest. Dermatol.125, 1190.
Geer, D.J., et al. 2005. Am. J. Pathol.167, 1575.
Hsu, YR., et al. 1999. Biochemistry38, 2523.
Qiao, J., et al. 1999. Development126, 547.
Werner, S., 1998. Cytokine Growth Factor Rev.9, 153.
Guo, L., et al. 1996. Genes Dev.10, 165.
Mason, I.J., et al. 1994. Mech. Dev.45, 15.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


存储类别

11 - Combustible Solids

wgk

WGK 2



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