427211
Lactate Dehydrogenase, Porcine Heart
别名:
Lactate Dehydrogenase, Porcine Heart
description
Merck USA index - 14, 5334
form
lyophilized
specific activity
≥300 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
impurities
≤0.01% PK, MK
≤0.03% GPT, GOT
≤0.1% MDH
pI
4.6
solubility
aqueous buffer: soluble
shipped in
ambient
storage temp.
−20°C
Quality Level
Disclaimer
Toxicity: Standard Handling (A)
General description
Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units
Other Notes
Kornberg, A. 1995. Methods Enzymol.1, 441
One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.
Preparation Note
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
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