444213
MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast
别名:
Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase
方案
≥90% (SDS-PAGE)
质量水平
表单
liquid
比活
≥600 mU/mg protein
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
溶解性
aqueous buffer: soluble
异质活性
other MMP activity, none detected
运输
wet ice
储存温度
−70°C
一般描述
Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.
Note: 1 mU = 1 milliunit.
包装
Please refer to vial label for lot-specific concentration.
外形
In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% Brij® 35 Detergent, 0.05% NaN₃, pH 7.0.
制备说明
Following initial thaw, aliquot and freeze (-70°C).
Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
其他说明
Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.
Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.
Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.
Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.
Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.
法律信息
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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