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Merck
CN

444213

Sigma-Aldrich

MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast

别名:

Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase

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关于此项目

UNSPSC代码:
12352202
NACRES:
NA.77
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方案

≥90% (SDS-PAGE)

质量水平

表单

liquid

比活

≥600 mU/mg protein

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

溶解性

aqueous buffer: soluble

异质活性

other MMP activity, none detected

运输

wet ice

储存温度

−70°C

一般描述

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.
Note: 1 mU = 1 milliunit.

包装

Please refer to vial label for lot-specific concentration.

外形

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% Brij® 35 Detergent, 0.05% NaN₃, pH 7.0.

制备说明

Following initial thaw, aliquot and freeze (-70°C).
Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

其他说明

Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.
Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.
Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

法律信息

Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Toxicity: Standard Handling (A)

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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