444232
MMP-9, Dimer, Human Neutrophil
别名:
Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase
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assay
≥90% (SDS-PAGE)
Quality Segment
form
liquid
specific activity
≥1000 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
foreign activity
other MMP activity, none detected
shipped in
wet ice
storage temp.
−70°C
General description
Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.
M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.
Physical form
In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% 35 Detergent, 0.05% NaN₃, pH 7.0.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Other Notes
Olson, M.W. et al. 2000. J. Biol. Chem.275, 2661.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
怎么回事？
WGK 1
存储类别
10 - Combustible liquids
闪点 (F)
Not applicable
闪点 (°C)
Not applicable