444235-M
MMP-13 Substrate, Fluorogenic
A quenched fluorescence substrate for MMP-13 (collagenase-3; kcat/Km = 1.09 x 10⁶ M⁻¹s⁻¹).
别名:
MMP-13 Substrate, Fluorogenic, Collagenase-3 Substrate, Fluorogenic
assay
≥98% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated, protect from light
color
yellow
solubility
DMSO: 5 mg/mL
fluorescence
λex ~325 nm, λem ~393 nm
storage temp.
−20°C
Quality Level
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General description
A quenched fluorescence substrate for MMP-13 (collagenase-3; kcat/Km = 1.09 x 106 M-1s-1; excitation maximum: ~325 nm; emission maximum: ~393 nm). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).
Biochem/physiol Actions
Primary Target
A quenched fluorescence substrate for MMP-13 (collagenase-3)
A quenched fluorescence substrate for MMP-13 (collagenase-3)
kcat/Km = 1.09 x 106 M⁻¹ sec⁻¹ for MMP-13 (collagenase-3)
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Knauper, V., et al. 1996. J. Biol. Chem. 271, 1544.
MCA-Pro-Cha-Gly-Nva-His-Ala-Dpa-NH₂ [Cha = L-cyclohexylalanine; Dpa = 3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl; Nva = L-norvaline]
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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