Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains an N-terminal propeptide which confers latency to the proenzyme, a Ca²⁺- and Zn²⁺- binding catalytic domain, a hinge region, and a C-terminal hemopexin domain. Hydrolyzes collagen type II 5-6 times faster than collagens type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2.

Note: 1 mU = 1 milliunit.

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

Following initial thaw, aliquot and freeze (-70°C).

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Pei, D., and Weiss, S.J. 1996. J. Biol. Chem.271, 9135.
Knight, C.G., et al. 1992. FEBS Lett.296, 263.

One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)