生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
AA1, monoclonal
表单
liquid
不包含
preservative
种属反应性
monkey, canine, human, feline
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (0.06-0.12 µg/mL)
Immunoblotting (1 µg/mL)
Paraffin Sections (0.06-0.12 µg/mL, heat pre-treatment required))
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TPSAB1(7177)
一般描述
Protein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.
Recognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.
This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.
免疫原
Human
native, human mast cell, tryptase
应用
Frozen Sections (0.06-0.12 µg/ml)
Immunoblotting (1 µg/ml)
Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
Immunoblotting (1 µg/ml)
Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
包装
Please refer to vial label for lot-specific concentration.
外形
In 10 mM PBS, pH 7.4.
制备说明
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
Cat, dog, human, or monkey mast cells
Cat, dog, human, or monkey mast cells
其他说明
Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75.
Little, S.S., and Johnson, D.A. 1995. Biochem. J.307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol.87, 111.
Walls, A.F., et al. 1990. J. Pathol.162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy20, 581.
Little, S.S., and Johnson, D.A. 1995. Biochem. J.307, 341.
Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol.87, 111.
Walls, A.F., et al. 1990. J. Pathol.162, 119.
Wall, A.F., et al. 1990. Clin. Exp. Allergy20, 581.
Staining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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