454849
MAP Kinase 1, His•Tag®, Human, Recombinant, E. coli
别名:
ERK1, MAPK3, p44 MAPK
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关于此项目
NACRES:
NA.54
UNSPSC Code:
12352202
Specific activity:
≥6000 units/mg protein
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥6000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.
Physical form
In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Stephanie Belanger-Jasmin et al.
Journal of neurochemistry, 103(5), 2022-2034 (2007-09-18)
Hairy/Enhancer of split (Hes) 6 is a basic helix-loop-helix protein that interacts with the transcriptional co-repressor, Groucho, and antagonizes the neural functions of the Notch pathway. More specifically, mouse Hes6 regulates cerebral corticogenesis by promoting neurogenesis and suppressing astrocyte differentiation.
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