475951
A22 hydrochloride
≥95% (HPLC),cell-permeable isothiourea compound, solid
别名:
MreB干扰化合物A22, S-（3,4-二氯苄基）异硫脲，HCI
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关于此项目
经验公式（希尔记法）:
C8H8Cl2N2S · xHCl
化学文摘社编号:
分子量:
235.13 (free base basis)
UNSPSC Code:
51111800
NACRES:
NA.77
MDL number:
产品名称
MreB干扰化合物A22, A cell-permeable isothiourea compound that specifically, rapidly and reversibly perturbs MreB function without affecting eukaryotic actin polymerization.
SMILES string
S(Cc1cc(c(cc1)Cl)Cl)\C(=N\[H])\N.Cl
InChI
1S/C8H8Cl2N2S.ClH/c9-6-2-1-5(3-7(6)10)4-13-8(11)12;/h1-3H,4H2,(H3,11,12);1H
InChI key
VBJNMXMOMSWRDV-UHFFFAOYSA-N
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
color
white
solubility
water: 2 mg/mL
DMSO: 200 mg/mL
shipped in
ambient
storage temp.
2-8°C
Quality Level
相关类别
Biochem/physiol Actions
主要靶标
MreB
MreB
产物不与ATP竞争。
可逆性：是
细胞可渗透性：具有
Disclaimer
毒性：标准处理（A）
General description
一种可渗透细胞的异硫脲化合物，可特异性，快速且可逆地干扰MreB功能，而不会影响真核肌动蛋白的聚合。据报告会影响新月柄杆菌的生长和形态，将杆状大肠埃希菌转化为圆形细胞，并在不影响DNA复制的情况下阻断DNA分离。建议与MreB′s核苷酸结合口袋相互作用，可用于MreB功能的时间分析。
Other Notes
Gitai, Z., et al. 2005.Cell120, 329.
Jensen, R.B., et al. 2002.Nat. Rev. Mol.Cell Biol.3, 167.
Iwai, N., et al. 2002.Biosci.Biotechnol.Biochem.66, 2658.
Jensen, R.B., et al. 2002.Nat. Rev. Mol.Cell Biol.3, 167.
Iwai, N., et al. 2002.Biosci.Biotechnol.Biochem.66, 2658.
Packaging
用惰性气体包装
Preparation Note
在DMSO中复溶后，分装并冷冻保存（-20°C）。DMSO贮备液在-20°C可稳定保存长达 1 个月。使用前在H₂O中复溶；贮备水溶液可稳定长达12小时。在酸性介质中可稳定保存2天。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Zemer Gitai et al.
Cell, 120(3), 329-341 (2005-02-15)
Faithful chromosome segregation is an essential component of cell division in all organisms. The eukaryotic mitotic machinery uses the cytoskeleton to move specific chromosomal regions. To investigate the potential role of the actin-like MreB protein in bacterial chromosome segregation, we
Noritaka Iwai et al.
Bioscience, biotechnology, and biochemistry, 66(12), 2658-2662 (2003-02-25)
Random screening for inhibitors of chromosome partitioning in Escherichia coli was done by the anucleate cell blue assay. A novel S-benzylisothiourea derivative, S-(3,4-dichlorobenzyl)isothiourea, tentatively named A22, was found to induce spherical cells and spherical anucleate cells in E. coli. Mecillinam
Rasmus B Jensen et al.
Nature reviews. Molecular cell biology, 3(3), 167-176 (2002-05-08)
A cellular differentiation programme that culminates in an asymmetric cell division is an integral part of the cell cycle in the bacterium Caulobacter crescentus. Recent work has uncovered mechanisms that ensure the execution of many events at different times during
Abhishek Shrivastava et al.
Science advances, 6(10), eaay6616-eaay6616 (2020-03-18)
The gliding bacterium Flavobacterium johnsoniae is known to have an adhesin, SprB, that moves along the cell surface on a spiral track. Following viscous shear, cells can be tethered by the addition of an anti-SprB antibody, causing spinning at 3
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