NEMO-Binding Domain Binding Peptide, Cell-Permeable

Cell permeable: yes

Primary Target
cytokine-induced NF-κB activation and NF-κB-dependent gene expression

Product does not compete with ATP.

Reversible: no

Toxicity: Standard Handling (A)

A cell-permeable Antennapedia-NBD (NEMO binding domain) (wild type) fusion peptide that exhibits anti-inflammatory activity in mouse model of acute inflammation. NBD is an amino terminal α-helical region of the NEMO (NF-κB essential modifier; IKKγ) associated with a carboxyl-terminal segment of IKKα and IKKβ. This peptide blocks the association of NEMO with the IKK complex and prevents NF-κB activation. NEMO is also reported to suppress the intrinsic basal activity of the IKK complex. Its anti-inflammatory properties are attributed to the inhibition of cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Does neither affect the TNF-α-induced phosphorylation of c-Jun nor the DNA binding of Oct-1 transcription factor.

A cell-permeable Antennapedia-NBD (wild type) fusion peptide that exhibits anti-inflammatory properties. Acts by inhibiting cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Suggested to block the interaction of NEMO with the IKK (IκB)-kinase complex and thereby prevents NF-κB activation. Does not affect the TNF-α-induced phosphorylation of c-Jun and DNA binding of the transcription factor Oct-1.

H-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Trp-Ser-Trp-Leu-Gln-Thr-Glu-OH

Jimi, E., et al. 2004. Nat. Med., 10, 617.
Siegmund, D., et al. 2001. J. Biol. Chem.276, 43708.
May, M.J., et al. 2000. Science289, 1550.
Li, Q., et al. 1999. Science284, 321.

Note: This peptide will precipitate when added to tissue culture medium even at 100 µM concentrations. However, sufficient material will stay in the medium to permeate cells.

Packaged under inert gas

Supplied as a trifluoroacetate salt.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany