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Merck
CN

482728

抗一氧化氮合酶(1131-1144),诱导型兔pAb

liquid, Calbiochem®

别名:

抗诱导型一氧化氮合酶

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
Citations:
17
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产品名称

抗一氧化氮合酶(1131-1144),诱导型兔pAb, liquid, Calbiochem®

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, rat, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Frozen Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... NOS1(4842)

Analysis Note

阳性对照
经IFNγ和LPS处理的原始264.7细胞

Application

冰冻切片(1:500)

免疫印迹(1:2000)

免疫细胞化学(1:500)

石蜡切片(1:500)

Disclaimer

毒性:标准处理(A)

General description

兔多克隆抗体以未稀释的血清形式提供。识别~130 kDa诱导型一氧化氮合酶(iNOS)蛋白。
识别 〜130 kDa iNOS 蛋白。
该抗一氧化氮合酶诱导型兔pAb经验证可用于冰冻切片、WB、ICC、石蜡切片,以检测诱导型一氧化氮合酶。

Immunogen

一种合成肽(CKKGSALEERKATRL),对应于与KLH结合的小鼠巨噬细胞诱导型一氧化氮合酶(iNOS)的氨基酸1131-1144
小鼠

Other Notes

Lowenstein, C., et al. 1992.Proc.Natl.Acad.Sci. USA89, 6711.
Lyons, C., et al. 1992.J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991.Nature351, 714.
与bNOS或eNOS无交叉反应。与测定条件相关的变量将决定适当的工作稀释度。



抗iNOS免疫细胞化学的推荐程序

:1.在适当的培养基中培养细胞。

2.通过用含有以下成分的新鲜培养基预处理细胞16小时来诱导NOS:不添加(对照),干扰素-γ(50 ng/ml),LPS(30 µg/ml)或干扰素加LPS。

3.用磷酸盐缓冲盐水(PBS)清洗细胞3-5分钟。

4.在室温下用2%皂苷渗透10分钟。

5.室温下在4%福尔马林中固定10分钟。

6.用PBS清洗3-5分钟。

7.在室温下用PBS中以1:500稀释的抗iNOS培养20分钟。

8.用PBS清洗3-5分钟。

9.用生物素化二抗(即生物素结合的山羊抗兔IgG,目录号401313)培养。清洗;与抗生物素蛋白结合的碱性磷酸酶(目录号189726或189732)一起培养。

10.用萘酚AS磷酸盐/固红紫色LB(目录号477758)或萘酚AS磷酸盐/新品红(目录号477759)展开。

Physical form

未稀释的血清。

Preparation Note

初次融化后,等分并冷冻(-20°C)。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1


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