产品名称
抗一氧化氮合酶(1131-1144),诱导型兔pAb, liquid, Calbiochem®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, rat, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1:500)
Immunoblotting (1:2000)
Immunocytochemistry (1:500)
Paraffin Sections (1:500))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NOS1(4842)
Analysis Note
阳性对照
经IFNγ和LPS处理的原始264.7细胞
经IFNγ和LPS处理的原始264.7细胞
Application
冰冻切片(1:500)
免疫印迹(1:2000)
免疫细胞化学(1:500)
石蜡切片(1:500)
免疫印迹(1:2000)
免疫细胞化学(1:500)
石蜡切片(1:500)
Disclaimer
毒性:标准处理(A)
General description
兔多克隆抗体以未稀释的血清形式提供。识别~130 kDa诱导型一氧化氮合酶(iNOS)蛋白。
识别 〜130 kDa iNOS 蛋白。
该抗一氧化氮合酶诱导型兔pAb经验证可用于冰冻切片、WB、ICC、石蜡切片,以检测诱导型一氧化氮合酶。
Immunogen
一种合成肽(CKKGSALEERKATRL),对应于与KLH结合的小鼠巨噬细胞诱导型一氧化氮合酶(iNOS)的氨基酸1131-1144
小鼠
Other Notes
Lowenstein, C., et al. 1992.Proc.Natl.Acad.Sci. USA89, 6711.
Lyons, C., et al. 1992.J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991.Nature351, 714.
Lyons, C., et al. 1992.J. Biol. Chem.267, 6370.
Bredt, D.S., et al. 1991.Nature351, 714.
与bNOS或eNOS无交叉反应。与测定条件相关的变量将决定适当的工作稀释度。
抗iNOS免疫细胞化学的推荐程序
:1.在适当的培养基中培养细胞。
2.通过用含有以下成分的新鲜培养基预处理细胞16小时来诱导NOS:不添加(对照),干扰素-γ(50 ng/ml),LPS(30 µg/ml)或干扰素加LPS。
3.用磷酸盐缓冲盐水(PBS)清洗细胞3-5分钟。
4.在室温下用2%皂苷渗透10分钟。
5.室温下在4%福尔马林中固定10分钟。
6.用PBS清洗3-5分钟。
7.在室温下用PBS中以1:500稀释的抗iNOS培养20分钟。
8.用PBS清洗3-5分钟。
9.用生物素化二抗(即生物素结合的山羊抗兔IgG,目录号401313)培养。清洗;与抗生物素蛋白结合的碱性磷酸酶(目录号189726或189732)一起培养。
10.用萘酚AS磷酸盐/固红紫色LB(目录号477758)或萘酚AS磷酸盐/新品红(目录号477759)展开。
抗iNOS免疫细胞化学的推荐程序
:1.在适当的培养基中培养细胞。
2.通过用含有以下成分的新鲜培养基预处理细胞16小时来诱导NOS:不添加(对照),干扰素-γ(50 ng/ml),LPS(30 µg/ml)或干扰素加LPS。
3.用磷酸盐缓冲盐水(PBS)清洗细胞3-5分钟。
4.在室温下用2%皂苷渗透10分钟。
5.室温下在4%福尔马林中固定10分钟。
6.用PBS清洗3-5分钟。
7.在室温下用PBS中以1:500稀释的抗iNOS培养20分钟。
8.用PBS清洗3-5分钟。
9.用生物素化二抗(即生物素结合的山羊抗兔IgG,目录号401313)培养。清洗;与抗生物素蛋白结合的碱性磷酸酶(目录号189726或189732)一起培养。
10.用萘酚AS磷酸盐/固红紫色LB(目录号477758)或萘酚AS磷酸盐/新品红(目录号477759)展开。
Physical form
未稀释的血清。
Preparation Note
初次融化后,等分并冷冻(-20°C)。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
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