一般描述
The STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.
应用
- An overnight (4°C) incubation is recommended for best results.
- This assay requires 25 μL diluted cell lysate per well.
- This kit must be run using Assay Buffer 1 (provided).
- 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Research Category
Signaling|Apoptosis & Cancer
Signaling|Apoptosis & Cancer
The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).
Used to detect/quantify:STAT1 (Tyr701)|STAT2 (Tyr690)|STAT3 (Tyr705)|STAT5A/B (Tyr694/699)|STAT6 (Tyr641)
生化/生理作用
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
包装
96-well plate
制备说明
Recommended storage for kit components is 2 - 8°C.
法律信息
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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