MILLIPLEX^® Protein Translation Signaling Panel

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

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For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Protein synthesis is required for cellular growth and development. Normal cells grow in a controlled manner in response to environmental and developmental cues. However, cancer cells can reprogram cellular metabolism, favoring uncontrolled growth and survival. This can be achieved by altering signaling pathways that control cellular processes such as protein synthesis.The phosphorylation state of proteins involved in translation initiation is a limiting factor that regulates the formation or activity of translational complexes. In cancer cells, hyper-activated signaling pathways influence translation, allowing uncontrolled growth and survival. In addition, several components of translation initiation have been found to be mutated, post-translationally modified, or differentially expressed, and thus have been shown to act as oncogenes.Translational alterations can increase the overall rate of protein synthesis as well as activate regulatory mechanisms leading to the translation of specific messenger RNAs for proteins that promote cancer progression and survival.Each MILLIPLEX^® cell signaling kit includes:• Stimulated and unstimulated cell lysates provided to qualify assay performance• Premixed magnetic beads to capture analytes of interest• Optimized detection antibody cocktails designed to yield consistent analyte profiles within a panelThe MILLIPLEX^® Protein Translation 6-plex Magnetic Bead kit is used to detect changes in phosphorylated eIF2a (Ser51), eIF-4B (Ser422), eIF-4E (Ser209), eIF-4G (Ser1108) and 4E-BP1 (Thr37/46), as well as total protein levels of 4E-BP1 in cell lysates using the Luminex^® system. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.

This panel targets phosphorylated proteins involved in the regulation of protein synthesis. It includes p70S6K (pT412), 4E-BP1 (pT37/46), eIF2α (pS51), eIF4E (pS209), mTOR (pS2448), and RPS6 (pS235/236). These proteins are downstream effectors of the mTOR pathway and play critical roles in translation initiation, cell growth, and metabolism. The panel supports studies in cancer, metabolic disorders, and cellular stress responses.

96-well plate

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MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 6 analytes in human, mouse, or rat cell lysate samples.Analytes Included: p70S6K (Phospho-Thr412), 4E-BP1 (Phospho-Thr37/46), eIF2α (Phospho-Ser51), eIF4E (Phospho-Ser209), mTOR (Phospho-Ser2448), RPS6 (Phospho-Ser235/236)Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).