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Merck
CN

487923

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3)

liquid, clone CC22.8C7.3, Calbiochem®

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
CC22.8C7.3, monoclonal
Application:
Citations:
13
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产品名称

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3), liquid, clone CC22.8C7.3, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

CC22.8C7.3, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity (predicted by homology)

all

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (2 µg/mL))

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... NOS1(4842)

General description

Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
Recognizes nitrotyrosine.
This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.

Analysis Note

Positive Control
Mouse macrophages treated with peroxynitrite

Application

Immunoblotting (2 µg/ml)

Disclaimer

Toxicity: Standard Handling (A)

Immunogen

peroxynitrite-treated KLH

Other Notes

Myatt, L., et al. 1996. Hypertension28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.

Preparation Note

Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Teresa Tiago et al.
Biochemistry, 45(11), 3794-3804 (2006-03-15)
Exposure of myosin subfragment 1 (S1) to 3-morpholinosydnonimine (SIN-1) produced a time-dependent inhibition of the F-actin-stimulated S1 Mg(2+)-ATPase activity, reaching 50% inhibition with 46.7 +/- 8.3 microM SIN-1 for 8.7 microM S1, that is, at a SIN-1/S1 molar ratio of
Enrique Samper et al.
Free radical biology & medicine, 46(3), 387-396 (2008-11-29)
Lymphomas adapt to their environment by undergoing a complex series of biochemical changes that are currently not well understood. To better define these changes, we examined the gene expression and gene ontology profiles of thymic lymphomas from a commonly used
Seungho Choi et al.
Neuroscience, 384, 41-53 (2018-05-22)
Alzheimer's disease (AD) is the most common form of dementia that is often accompanied by mood and emotional disturbances and seizures. There is growing body of evidence that neurons expressing γ-aminobutyric acid (GABA) play an important role in regulation of
Sanjeev Kumar et al.
The Journal of biological chemistry, 279(51), 53475-53482 (2004-10-01)
Plasmodium berghei invasion of Anopheles stephensi midgut cells causes severe damage, induces expression of nitric-oxide synthase, and leads to apoptosis. The present study indicates that invasion results in tyrosine nitration, catalyzed as a two-step reaction in which nitric-oxide synthase induction
A R Jayakumar et al.
Neuroscience, 218, 305-316 (2012-05-23)
Brain edema is an important complication of acute hepatic encephalopathy (AHE), and astrocyte swelling is largely responsible for its development. Elevated blood and brain ammonia levels have been considered as major etiological factors in this edema. In addition to ammonia

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