生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
CC22.8C7.3, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性（根据同源性预测）
all
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (2 µg/mL))
同位素/亚型
IgG2b
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... NOS1(4842)
一般描述
Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
Recognizes nitrotyrosine.
This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.
免疫原
peroxynitrite-treated KLH
应用
Immunoblotting (2 µg/ml)
外形
In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.
制备说明
Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
分析说明
Positive Control
Mouse macrophages treated with peroxynitrite
Mouse macrophages treated with peroxynitrite
其他说明
Myatt, L., et al. 1996. Hypertension28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Sanjeev Kumar et al.
The Journal of biological chemistry, 279(51), 53475-53482 (2004-10-01)
Plasmodium berghei invasion of Anopheles stephensi midgut cells causes severe damage, induces expression of nitric-oxide synthase, and leads to apoptosis. The present study indicates that invasion results in tyrosine nitration, catalyzed as a two-step reaction in which nitric-oxide synthase induction
Seungho Choi et al.
Neuroscience, 384, 41-53 (2018-05-22)
Alzheimer's disease (AD) is the most common form of dementia that is often accompanied by mood and emotional disturbances and seizures. There is growing body of evidence that neurons expressing γ-aminobutyric acid (GABA) play an important role in regulation of
Teresa Tiago et al.
Biochemistry, 45(11), 3794-3804 (2006-03-15)
Exposure of myosin subfragment 1 (S1) to 3-morpholinosydnonimine (SIN-1) produced a time-dependent inhibition of the F-actin-stimulated S1 Mg(2+)-ATPase activity, reaching 50% inhibition with 46.7 +/- 8.3 microM SIN-1 for 8.7 microM S1, that is, at a SIN-1/S1 molar ratio of
Enrique Samper et al.
Free radical biology & medicine, 46(3), 387-396 (2008-11-29)
Lymphomas adapt to their environment by undergoing a complex series of biochemical changes that are currently not well understood. To better define these changes, we examined the gene expression and gene ontology profiles of thymic lymphomas from a commonly used
Mathews Valuparampil Varghese et al.
American journal of physiology. Lung cellular and molecular physiology, 320(4), L508-L521 (2021-01-28)
We have previously reported that several patients with idiopathic pulmonary hypertension (PH) had different types of G6PD deficiency. However, the role of G6PD in PH is multifactorial because G6PD is involved in controlling oxidative stress, metabolic switch, and red blood
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