biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
CC22.8C7.3, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (2 µg/mL))
isotype
IgG2b
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NOS1(4842)
General description
Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
Recognizes nitrotyrosine.
This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.
Immunogen
peroxynitrite-treated KLH
Application
Immunoblotting (2 µg/ml)
Physical form
In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.
Preparation Note
Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Mouse macrophages treated with peroxynitrite
Mouse macrophages treated with peroxynitrite
Other Notes
Myatt, L., et al. 1996. Hypertension28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
怎么回事？
WGK 1
闪点 (F)
Not applicable
闪点 (°C)
Not applicable