Phosphodiesterase 3B, His•Tag^®, Human, Recombinant, S. frugiperda

Full-length, recombinant, human phosphodiesterase 3B (accession no. AY459346) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. An important component of insulin and cAMP-dependent signaling. Supplied as a partially purified preparation.

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Following initial thaw, aliquot and freeze (-70°C).

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Kitamura, T., et al. 1999. Mol. Cell. Biol.19, 6286.
Degerman, E., et al. 1997. J. Biol. Chem.272, 6823.

One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)