recombinant
expressed in baculovirus infected Sf21 cells
form
liquid
specific activity
≥0.25 U/μL
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
Quality Level
General description
Full-length, recombinant, human, phosphodiesterdase 10A2 (accession no. 10A2) fused at the N-terminus to a His•Tag7reg; sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of catalytically active enzyme.
Physical form
In RIPA buffer (150 mM NaCl, 10 mM Tris-HCl, 1% NP-40), pH 8.3.
Other Notes
Kotera, J., et. al. 2004. J. Biol. Chem.279, 4366.
Fujishige K., et. al. 1999. Eur. J. Biochem.266, 1118.
Kotera, J., et. al. 1999. Biochem. Biophys. Res. Commun.261, 551.
Fujishige K., et. al. 1999. Eur. J. Biochem.266, 1118.
Kotera, J., et. al. 1999. Biochem. Biophys. Res. Commun.261, 551.
One unit is defined as the amount of enzyme required to convert 1 µmol cAMP to AMP per min at 30°C, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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