biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr)
Quality Level
Gene Information
human ... PID1(55022)
General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes phosphotyrosine-containing proteins.
Recognizes a broad spectrum of tyrosine-phosphorylated proteins in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Phosphotyrosine.
Immunogen
purified phosphotyrosine conjugated to KLH
Application
ELISA (1:5000-1:10,000)
Immunoblotting (1:1000-1:2000)
Immunoprecipitation (2-5 µg)
Immunoblotting (1:1000-1:2000)
Immunoprecipitation (2-5 µg)
Packaging
Please refer to vial label for lot-specific concentration.
Other Notes
Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. Monospecific for phosphotyrosine. Little or no cross-reactivity with phosphothreonine- or phosphoserine-containing proteins. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Fabienne Chausson et al.
Development, growth & differentiation, 46(1), 71-82 (2004-03-11)
The molecular mechanisms underlying oocyte maturation in the annelid polychaetes Arenicola marina and Arenicola defodiens were investigated. In both species, a hitherto unidentified hormone triggers synchronous and rapid transition from prophase to metaphase, a maturation process which can be easily
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