生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性(根据同源性预测)
all
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pTyr)
基因信息
human ... PID1(55022)
一般描述
Immunoaffinity purified rabbit polyclonal antibody. Recognizes phosphotyrosine-containing proteins.
Recognizes a broad spectrum of tyrosine-phosphorylated proteins in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Phosphotyrosine.
免疫原
purified phosphotyrosine conjugated to KLH
应用
ELISA (1:5000-1:10,000)
Immunoblotting (1:1000-1:2000)
Immunoprecipitation (2-5 µg)
Immunoblotting (1:1000-1:2000)
Immunoprecipitation (2-5 µg)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
其他说明
Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. Monospecific for phosphotyrosine. Little or no cross-reactivity with phosphothreonine- or phosphoserine-containing proteins. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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